Correlation of sperm DNA integrity with other semen parameters and analyse the role of abnormal sperm DNA in evaluation of infertility

Abstract

Semen analysis forms the base line investigation and its results are taken as a surrogate measure of male fecundity. Aim and Objective: To assess the prevalence of abnormal sperm DNA integrity index and to correlate it with the other semen parameters and analyse its role as an efficient tool in evaluation of infertility. Material and Methods: Samples were collected from men visiting for evaluation of infertility. Routine semen parameter was assessed as per WHO manual 5TH Edition and abnormal sperm DNA integrity index was calculated using Toludine blue method. Samples were grouped into men with normal semen parameter and men with abnormal semen parameters based on WHO 2010 criteria. Sperm DNA integrity index was then correlated with the routine semen parameters and also within the study groups. Results: Abnormal sperm DNA integrity index showed a significant negative correlation with sperm count, motility and morphology.