Achyranthes aspera extract against Pseudomonas fluorescens enhance antibody production in Labeo rohita

Abstract

Indian major carp, rohu, (Labeo rohita) (30±6g), were fed with two types of diets; an experimental diet, containing root extract (0.5%) of Achyranthes aspera as an ingredient and control diet without the root extract. Experimental rohu were injected intraperitoneally with Pseudomonas fluorescens (3.2×10-6 CFU ml-1) and bloods were sampled 14 and 28 days post immunization. Though hemagglutination antibody (HA) titers were always higher in the test group than the control group, antigen-specific antibody and total serum globulin levels peaked on day 14 after immunization and steadily reduced towards day 28. Beside this, the results showed that dietary supplementation of A. aspera in test group showed significantly increased serum antibody titer (3280) and growth factors (41.1%) of L. rohita (P