Achyranthes aspera Extract as feed additives enhances immunological parameters and growth performance in Pangasius pangasius against Pseudomonas fluorescens

Abstract

Pangus (Pangasius pangasius) has been increasing for its high potential and play vital role in aquaculture in Bangladesh. The effects of dietary A. aspera supplementation on innate immunity, growth and disease against P. fluorescens infection were evaluated in Pangus (P. pangasius). Fish were feeding for 28 days with control diet and three experimental diets containing A. aspera herbal diet which were prepared with at 0%, 0.5%, 1% and 1.5% of root extract respectively. Various innate immune parameters examined at 14 and 28 days post-feeding. All four Groups of pangus injected intraperitoneally with P. fluorescens (3.2×10-6 CFU ml-1) and after 14 and 28 days of post immunization, bloods sampled collected from live fish with a hypodermic syringe. The present results demonstrated that Dietary supplementation of A. aspera (1.5%) showed significantly increased serum antibody titer (3280), phagocytic activity (49%), immune response as both specific (44%) and non-specific (3%), activation of disease resistance and growth factors (41.1%) of P. pangasius (P