articleTop 1% cited
SHERLOCK: nucleic acid detection with CRISPR nucleases
Nature Protocols · 2019 · Vol. 14(10) · pp. 2986–3012
Max J. Kellner✉(Broad Institute)Jeremy Koob(Broad Institute)Jonathan S. Gootenberg(Broad Institute)Omar O. Abudayyeh(Broad Institute)Feng Zhang(Broad Institute)
CRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionCRISPRTrans-activating crRNANucleic acidRecombinase Polymerase AmplificationMultiplexDNAComputational biologyRNALoop-mediated isothermal amplificationNucleic acid detection
MeSH terms
Zika Virus InfectionZika VirusRNA, Guide, CRISPR-Cas SystemsEndonucleasesHumansNucleic AcidsRecombinant ProteinsRibonucleasesProtein EngineeringRNA, Guide, KinetoplastidaDNA PrimersNucleic Acid Amplification TechniquesLeptotrichiaWorkflowCRISPR-Cas Systems
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References
Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
BMC Bioinformatics · 2012 · 6,131 citations
Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products
Nature Protocols · 2008 · 1,837 citations
DNA Detection Using Recombination Proteins
PLoS Biology · 2006 · 2,380 citations
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Cell · 2015 · 4,775 citations
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