article Open AccessTop 1% cited
Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
BMC Bioinformatics · 2012 · Vol. 13(1) · pp. 134–134
Jian Ye✉(National Institutes of Health)George Coulouris(National Center for Biotechnology Information)Irena Zaretskaya(National Institutes of Health)Ioana Cutcutache(Duke-NUS Medical School)Steve Rozen(Duke-NUS Medical School)Thomas Madden(National Center for Biotechnology Information)
Abstract
We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties. This tool is publicly available at http://www.ncbi.nlm.nih.gov/tools/primer-blast.
Molecular Biology Techniques and ApplicationsGenomics and Phylogenetic StudiesRNA and protein synthesis mechanismsPrimer (cosmetics)Polymerase chain reactionComputer scienceComputational biologyIn silico PCRPrimer dimerBiologyGeneticsMultiplex polymerase chain reactionGene
MeSH terms
AlgorithmsCarrier ProteinsHumansIntronsMolecular Sequence DataSoftwarePolymerase Chain ReactionDNA PrimersPolymorphism, Single Nucleotide
Funding
- Agency for Science, Technology and Research
- National Institutes of Health
- U.S. National Library of Medicine
Citations
6,131
FWCI
76.11
field-weighted impact
References
14
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100%
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References
A general method applicable to the search for similarities in the amino acid sequence of two proteins
Journal of Molecular Biology · 1970 · 11,386 citations
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Nucleic Acids Research · 1997 · 74,154 citations
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