Performance evaluation of chaetoglobosin against fruit rot disease on chilli

Abstract

Chaetoglobosin is a biomolecule produced by the fungus Chaetomium globosum. In the present study Chaetomium isolate Ch-1 was isolated from Solanum tuberosum and used for the extraction of secondary metabolite named as Chaetoglobosin. The extracted metabolite was injected in HPLC for confirmation and showed the retention time between between 7.51 and 7.53 min which is matching with the retention time of chaetoglobosin standard obtained from Sigma Aldrich. Co.LLC, USA. The efficacy of chaetoglobosin was tested against Colletotrichum capsici causing fruit rot disease on chilli under in vitro and glass house conditions. Chaetoglobosin at 2000 ppm concentration recorded 67.14 per cent inhibition of mycelial growth whereas at 1500 ppm showed 52.87 per cent mycelial inhibition over control. Among the different concentrations, chaetoglobosin at 2000 ppm showed 53.31 per cent inhibition of total biomass production against C. capsici. In the in vivo, chaetglobosin applied both individually and in combination with azoxystrobin and tebuconazole fungicides. The individual application of chaetoglobosin at 0.2 per cent concentration showed 56.78 per cent disease reduction over control. The combination of azoxystrobin (Willowood) with chaetoglobosin exhibited 81.82 per cent reduction of (10.69 PDI) fruit rot incidence. Tebuconazole and chaetoglobosin combination showed 76.73 per cent disease reduction (13.24 PDI) over control.