Study in vitro evaluation of plant extracts and fungicides against A. cucumerina var. cyamopsidis. of cluster bean

Abstract

Culture media on growth of the fungus as well as colony characteristics of the isolated Alternaria from diseased Cluster bean leaves is needed to be developed for suitable management strategies of the disease and also knowledge of nutritional pattern and factors influencing the growth of the fungus. In In vitro bioassay of botanicals, total 15 treatments/botanicals including control were evaluated against A. c. var. cyamopsidis in-vitro conditions at @ 10% and 20% concentration. Maximum percentage inhibition was found using Allium cepa (75.41% and 100%), P. hysterophorous (68.73% and 100%) on both concentration after 7 days of inoculation while C. fistula showed the maximum inhibition after 3 days of inoculation. Minimum percentage inhibition was revealed by Lantana camara (31.83% and 45.38%) at both concentrations. In vitro bioassay of fungicides, total eleven treatments/fungicides including control were evaluated against A.c. var. cyamopsidis in-vitro conditions at 0.1 per cent and 0.2 per cent concentrations. Maximum percentage inhibition was found using Mancozeb (100% and 100%), Carbendazim (100% and 100%), Tebuconazole (91.72% 100%), Thiophanate methyl (100% and 100%), and Vitavax (60.32% and 85.76%) showed maximum inhibtion only after 3 and 5 days of inoculation. Minimum percentage inhibition was seen by Tricyclazole (32.96% and 55.91%) at both concentrations. The complete inhibition was found using Allium cepa and P. hysterophorous at 20% concentration under in vitro conditions. Complete inhibition was found using Mancozeb, Carbendazim, and Thiophanate methyl at 0.1 per cent concentration under in vitro conditions.