Western Ghats terrestrial microalgae serve as a source of amylase and antioxidants enzymes

Abstract

The purpose of the study was to screen six identified microalga samples from in and around of the Western Ghats Karnataka state for their amylase and antioxidants activity assay. These six collected terrestrial microalgae were grown in plastic tray for multiplication purpose treated with R.O. residual dispense water, pH 8.4 (T1) and urban waste water (T2)­­­ which reduced the pH and TDS content in that water. Rhizoclonium hieroglyphicum has greater chlorophyll content, which helps in multiplication of microalgae very quickly. Carbohydrate degrading enzymes were analyzed using DNS reagents. Free radical scavenging activities were also determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) method. Superoxide Dismutase and Peroxidase was the most important antioxidant enzymes that protect against the peroxidation system and maintain the redox state of the cell. Amylase, SOD and POD activities were confirmed by conducting native PAGE.