In vitro shoot bud induction in Guggul [Commiphora wightii (Arnott)]

Abstract

Shoot apex and nodal segment explants of guggul were placed on Murashige and Skoog Medium (MS medium, 1962) supplemented with different concentration of cytokinins and auxins alone or in combination for shoot bud induction. In shoot apex explants maximum shoot bud induction was observed on a medium containing 3.5 mg/l BAP with 90 per cent frequency followed by 4.5 mg/l Kn+1.0 mg/l IAA with 70 per cent frequency. Whereas, maximum shoot bud induction from nodal segment explant was observed on a medium containing 3.0 mg/l BAP with 90 per cent frequency followed by 4.5 mg/l BAP+1.0 mg/l IAA with 70 per cent frequency.