In vitro study of medicinally important orchid Aerides multiflora Roxb. from nodal and leaf explants

Abstract

An efficient procedure described for mass propagation of Aerides multiflora Roxb. using in vitro sourced nodal and leaf explants. Regeneration response varied with the explants, nutrient compositions and PGRs. Nodal segments responded the best than leaf segments. In vitro grown nodal segments directly produced highest average number of multiple shoot buds (8.83 ± 0.45/segment) via organogenesis sprouted on MS medium with 1.0 mg/l NAA + 2.0 mg/l BAP followed by (8.65 ± 0.54/segment) MS + 0.5 mg/l NAA + 1.0 mg/l BAP. Leaf segments underwent proliferation via embryogenesis and produced maximum per cent of greenish PLBs on MS medium with 1.0 mg/l IAA + 2.0 mg/l BAP followed by MS + 1.0 mg/l NAA + 2.0 mg/l BAP. Highest increased individual shoot bud length was achieved on agar solidified MS with 1.0 mg/l NAA + 1.0 mg/l BAP (2.62 ± 0.14 cm) followed by liquid MS + 1.0 mg/l IAA + 1.0 mg/l BAP (2.53 ± 0.11 cm) medium. Elongation of shoot bud was better in solidified media than liquid condition and MS was superior than PM. Increase in length as well as the number of roots developed MSBs derived seedlings were best responses on agar solidified MS with 1.0 mg/l IBA (4.32 ± 0.24 cm/shoot bud and 3.19 ± 0.22 no/shoot bud) followed by MS + 0.5 mg/l IAA (3.91 ± 0.23 cm/shoot bud and 3.01 ± 0.16 no/shoot bud). The plantlets were hardened in vitro for 3-4 wks prior to transfer in green house.