In vitro callus induction from different explants in Pomegranate (Punica granatum L.)

Abstract

Leaf, Shoot apex and Nodal segment explant of pomegranate were placed on Murashige and Skoog Medium (MS medium, 1962) supplemented with different concentration of cytokinins and auxins for callus induction. Maximum callus induction was observed on a medium containing 1.5 mg/l BAP followed by 1.5 mg/l Kn and IAA in leaf explant with 100 per cent frequency. In shoot apex explants, maximum callus induction was observed on 2.0 mg/l BAP followed by 2.0 mg/l Kn with 100 per cent frequency. Maximum callus induction in Nodal segment was observed on 2.0 mg/l BAP followed by 2.0 mg/l IAA with 100 per cent frequency.