Biological activities of Amoora rohituka Roxb. Leaf extracts through dose-mortality, insect repellency, cytotoxicity, larvicidal and antimicrobial assays

Abstract

Biological activities of Petroleum ether (Pet. ether), CHCl3, CH3OH and EtOAc extracts of Amoora rohituka leaf were tested against the red flour beetle Tribolium castaneum (Hbst.) adults for dose-mortality and insect repellent activity; against eggplant aphid Aphis gossypii Glover for repellent activity; against brine shrimp Artemia salina L. nauplii for cytotoxicity; against larvae of Culex quinquefasciatus Say for larvicidal activity; and against five bacteria and fungi for anti-microbial activity. The dose-mortality observed for Pet. ether, EtOAc and CHCl3 extracts with LD50 values 1.769, 1.049, 0.871 and 0.249mg cm-2; 2.099, 1.047, 0.927 and 0.819mg cm-2; and 2.847, 2.126, 2.053, 1.806mg cm-2 for 12, 24, 36 and 48h of exposure respectively, whereas CH3OH extract did not show any mortality against the adult beetles of T. castaneum. The CHCl3, CH3OH and EtOAc extracts showed promising repellent activity against T. castaneum adults at P