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Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Nature Biotechnology · 2017 · Vol. 35(8) · pp. 725–731

Robert M. Bowers✉(Joint Genome Institute)Nikos C. Kyrpides(Joint Genome Institute)Ramūnas Stepanauskas(Bigelow Laboratory for Ocean Sciences)Miranda Harmon‐Smith(Joint Genome Institute)Devin F. R. Doud(Joint Genome Institute)T. B. K. Reddy(Joint Genome Institute)Frederik Schulz(Joint Genome Institute)Jessica K. Jarett(Joint Genome Institute)Adam R. Rivers(Agricultural Research Service)Emiley A. Eloe‐Fadrosh(Joint Genome Institute)Susannah G. Tringe(University of California, Merced)Natalia Ivanova(Joint Genome Institute)Alex Copeland(Joint Genome Institute)Alicia Clum(Joint Genome Institute)Eric D. Becraft(Bigelow Laboratory for Ocean Sciences)Rex R. Malmstrom(Joint Genome Institute)Bruce W. Birren(Broad Institute)Mircea Podar(Oak Ridge National Laboratory)Peer Bork(European Molecular Biology Laboratory)George M. Weinstock(Jackson Laboratory)George M Garrity(Michigan State University)Jeremy A. Dodsworth(California State University, San Bernardino)Shibu Yooseph(J. Craig Venter Institute)Granger Sutton(J. Craig Venter Institute)Frank Oliver Glöckner(Max Planck Institute for Marine Microbiology)Jack A. Gilbert(Argonne National Laboratory)William Nelson(Pacific Northwest National Laboratory)Steven Hallam(University of British Columbia)Sean P. Jungbluth(University of Southern California)Thijs J. G. Ettema(Uppsala University)Scott Tighe(University of Vermont)Konstantinos T. Konstantinidis(Georgia Institute of Technology)Wen‐Tso Liu(University of Illinois Urbana-Champaign)Brett J. BakerThomas Rattei(University of Vienna)Jonathan A. Eisen(University of California, Davis)Brian P. Hedlund(University of Nevada, Las Vegas)Katherine D. McMahon(University of Wisconsin–Madison)Noah Fierer(Cooperative Institute for Research in Environmental Sciences)Rob Knight(University of California San Diego)ROBERT FINN(European Bioinformatics Institute)Guy Cochrane(European Bioinformatics Institute)Ilene Karsch‐Mizrachi(National Institutes of Health)Gene W. Tyson(The University of Queensland)Christian Rinke(The University of Queensland)Alla Lapidus(St Petersburg University)Folker Meyer(Argonne National Laboratory)Pelin Yilmaz(Max Planck Institute for Marine Microbiology)Donovan H. Parks(The University of Queensland)A. Murat Eren(University of Chicago)Lynn M. Schriml(National Cancer Institute)Jillian F. Banfield(Planetary Science Institute)Philip Hugenholtz(The University of Queensland)Tanja Woyke(University of California, Merced)

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Abstract

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Genomics and Phylogenetic Studies Microbial Community Ecology and Physiology Gut microbiota and healthMetagenomicsArchaeaGenomeBacteriaBiologyComputational biologyGeneticsBacterial genome sizeBacterial geneticsGene