In Vitro study on Antiglycation activity, Antioxidant activity and Phenolic content of Osbeckia octandra L. leaf decoction

Abstract

Non enzymatic glycation is the major cause of spontaneous damage to proteins leading to various complications due to formation of non-reversible Advanced Glycation end Products (AGEs) and oxidative stress. Medicinal plants having both antiglycation and antioxidant activity may be good therapeutic potential in the treatment of diabetic mellitus. The present study was undertaken to investigate the antiglycation effect, antioxidant activity and phenolic content of Osbeckia octandra leaf decoction used in the treatment of diabetes mellitus in Ayurvedic medicine. The water extracts of the three samples of Osbeckia octandra leaves from three different areas had significant anti-glycation activity as 23.0 µg/mL, 25.2 µg/mL, 28.5 µg/mL, ABTS antioxidant activity as 1375, 794, 1231 TEAC mmol/g and DPPH activity as 55.5 µg/mL, 97.7 µg/mL, and 98.4 µg/mL respectively. Total phenolic content was from 483-667 mg/GAE/g.