Evaluation of the Main Polyphenolic Compounds in Aromatic Plants of Asteraceae and Solanaceae Families of Bulgarian Origin

Abstract

Bulgarian specimens of Asteraceae and Solanaceae families are very scarce studied concerning the polyphenolic compounds. The aim of this investigation was to obtain fingerprint chromatographic profiles and to quantify the main polyphenols. HPLC–PDA on Kromasil and Purospher columns was employed for the separation of the components by applying two methods (TFA and AcAc). Better separation and reliable quantification of the polyphenols in Achillea species were obtained by the TFA method on Kromasil column. Chromatographic profiling and quantification of polyphenols in A. campestris was successful by using the TFA method on both columns. The polyphenols of A. vulgaris were better separated by AcAc method on Purospher column. Caffeoylquinic acids and rutin were present in higher quantities in Achillea asplenifolia and tobacco (Djebel). Fingerprint chromatographic profiles and reliable quantification of the polyphenolic compounds were achieved by considering the differences in the polyphenol complex and the specificity of the plant matrix.