article Open AccessTop 1% cited
Genome Engineering of <i>Drosophila</i> with the CRISPR RNA-Guided Cas9 Nuclease
Genetics · 2013 · Vol. 194(4) · pp. 1029–1035
Scott J. Gratz(University of Wisconsin–Madison)Alexander M Cummings(University of Wisconsin–Madison)Jennifer Nguyen(University of Wisconsin–Madison)Danielle C. Hamm(University of Wisconsin–Madison)Laura K. Donohue(University of Wisconsin–Madison)Melissa M. Harrison✉(University of Wisconsin–Madison)Jill Wildonger✉(University of Wisconsin–Madison)Kate M. O’Connor-Giles✉(University of Wisconsin–Madison)
Abstract
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
CRISPR and Genetic EngineeringChromosomal and Genetic VariationsInsect symbiosis and bacterial influencesBiologyCRISPRNucleaseGeneticsCas9Genome engineeringGenome editingGenomeRNADrosophila (subgenus)
MeSH terms
AnimalsDrosophilaEndodeoxyribonucleasesGenetic EngineeringGerm-Line MutationGene TargetingDrosophila ProteinsGenome, InsectHomologous RecombinationClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas Systems
Funding
- National Institutes of Health
Citations
1,052
FWCI
52.18
field-weighted impact
References
33
Percentile
100%
vs. same field & year
Citations per year
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References
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