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Transcriptome sequencing in an ecologically important tree species: assembly, annotation, and marker discovery

BMC Genomics · 2010 · Vol. 11(1) · pp. 180–180
Thomas L. ParchmanKatherine S. GeistJohan A. GrahnenCraig W. BenkmanC. Alex Buerkle

Abstract

This sequence collection represents a major genomic resource for P. contorta, and the large number of genetic markers characterized should contribute to future research in this and other pines. Our results illustrate the utility of next generation sequencing as a basis for marker development and population genomics in non-model species.

Genomics and Phylogenetic StudiesMolecular Biology Techniques and ApplicationsPlant Disease Resistance and GeneticsBiologyContigPinus contortaGenomeGeneticsExpressed sequence tagComputational biologyDNA sequencingGenomicsEvolutionary biology

MeSH terms

Genetic MarkersTreesGenes, PlantSequence Analysis, DNADNA PrimersDNA, ComplementaryMinisatellite RepeatsRetroelementsDNA, PlantGenome, PlantExpressed Sequence TagsContig MappingPolymorphism, Single NucleotideGene Expression ProfilingGenomics

Funding

  • National Science Foundation
  • University of Wyoming
  • National Institutes of Health
  • Division of Biological Infrastructure
  • Division of Environmental Biology
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