article Open AccessTop 1% cited
Accuracy and quality of massively parallel DNA pyrosequencing
Genome biology · 2007 · Vol. 8(7) · pp. R143–R143
Susan M. Huse✉Julie A. Huber(Marine Biological Laboratory)Hilary G. Morrison(Marine Biological Laboratory)Mitchell L. Sogin(Marine Biological Laboratory)David B. Mark Welch(Marine Biological Laboratory)
Abstract
By using objective criteria to eliminate low quality data, the quality of individual GS20 sequence reads in molecular ecological applications can surpass the accuracy of traditional capillary methods.
Microbial Community Ecology and PhysiologyProtist diversity and phylogenyEnvironmental DNA in Biodiversity StudiesPyrosequencingBiologyMassive parallel sequencingHuman geneticsComputational biologyMassively parallelDNA sequencingDNAGeneticsEvolutionary biology
MeSH terms
DNA, RibosomalGenetics, MicrobialGenetic VariationReproducibility of ResultsGenomeSequence Analysis, DNAGenes, rRNA
Funding
- National Science Foundation
- National Aeronautics and Space Administration
- W. M. Keck Foundation
- National Institutes of Health
- National Institute of Environmental Health Sciences
Citations
1,295
FWCI
72.73
field-weighted impact
References
23
Percentile
100%
vs. same field & year
Citations per year
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