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“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

Analytical Biochemistry · 1981 · Vol. 112(2) · pp. 195–203

W. Neal Burnette✉(Fred Hutch Cancer Center)

RNA and protein synthesis mechanisms Bacteriophages and microbial interactions Advanced Proteomics Techniques and ApplicationsNitrocelluloseSodium dodecyl sulfateBlotChemistryPolyacrylamideChromatographyPolyacrylamide gel electrophoresisElectrophoresisSodiumGel electrophoresis

MeSH terms

AnimalsAntigens, ViralAutoradiographyCapsidChromatography, GelCollodionElectrophoresisElectrophoresis, Polyacrylamide GelImmunologic TechniquesMaleMethodsLeukemia Virus, MurineSodium Dodecyl SulfateStaphylococcal Protein AMice

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References

Handbook of Experimental Immunology.

Annals of Internal Medicine · 1968 · 2,420 citations

High resolution two-dimensional electrophoresis of proteins.

Journal of Biological Chemistry · 1975 · 19,355 citations

An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates

European Journal of Biochemistry · 1976 · 3,966 citations

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HANDBOOK OF EXPERIMENTAL IMMUNOLOGY

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