articleTop 1% cited
A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots
Analytical Biochemistry · 1984 · Vol. 136(1) · pp. 175–179
M. S. Blake✉(Rockefeller University)Kenneth H. Johnston(Rockefeller University)G.J. Russell-Jones(Rockefeller University)E C Gotschlich(Rockefeller University)
Advanced biosensing and bioanalysis techniquesMonoclonal and Polyclonal Antibodies ResearchBiosensors and Analytical DetectionNitrocelluloseAlkaline phosphataseChemistryWestern blotMolecular biologyBlotAntiserumAntibodyAntigenCoomassie Brilliant Blue
MeSH terms
Alkaline PhosphataseAnimalsAntibodiesAntigen-Antibody ComplexAntigens, BacterialCollodionElectrophoresisHistocytochemistryNeisseria gonorrhoeaeNerve Tissue ProteinsRabbitsSynapsins
Funding
- National Institutes of Health
Citations
2,149
FWCI
33.74
field-weighted impact
References
15
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100%
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Citations per year
References
Refinement of the Coomassie blue method of protein quantitation
Analytical Biochemistry · 1978 · 1,585 citations
The use of tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes
Journal of Immunological Methods · 1982 · 557 citations
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature · 1970 · 251,243 citations
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
Analytical Biochemistry · 1981 · 8,483 citations
Enzyme-Linked Immunosorbent Assay, Elisa
The Journal of Immunology · 1972 · 1,743 citations
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