articleTop 1% cited
The use of tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes
Journal of Immunological Methods · 1982 · Vol. 55(3) · pp. 297–307
Byron E. Batteiger✉(Indiana University School of Medicine)W J Newhall(Indiana University – Purdue University Indianapolis)Robert B. Jones(Indiana University – Purdue University Indianapolis)
Protein purification and stabilityMonoclonal and Polyclonal Antibodies ResearchAntimicrobial Peptides and ActivitiesNitrocelluloseSodium dodecyl sulfateElectroblottingMembrane proteinStainingAntiserumPolyacrylamide gel electrophoresisAntigenChemistryChromatography
MeSH terms
AnimalsAntigens, BacterialAutoradiographyBacterial ProteinsBinding, CompetitiveCelluloseChlamydia trachomatisElectrophoresis, Polyacrylamide GelImmunologic TechniquesMembranes, ArtificialNeisseria gonorrhoeaePolysorbatesRabbits
Funding
- National Institutes of Health
Citations
557
FWCI
17.46
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References
17
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Cited by
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References
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FEBS Letters · 1977 · 925 citations
Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril
Biochemical and Biophysical Research Communications · 1978 · 4,594 citations
A rapid, sensitive, and specific method for the determination of protein in dilute solution
Analytical Biochemistry · 1973 · 2,523 citations
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature · 1970 · 251,243 citations
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
Analytical Biochemistry · 1981 · 8,483 citations
A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding
Analytical Biochemistry · 1976 · 225,297 citations
Immunologic Relationship Between Genital Tric, Lymphogranuloma Venereum, and Related Organisms in a New Microtiter Indirect Immunofluorescence Test
American Journal of Ophthalmology · 1970 · 539 citations
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Analytical Biochemistry · 1976 · 209,807 citations
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