Enzyme method for determination of α‐linked glucose polymers in biological materials

Abstract

Abstract A review of methods available for the determination of starch suggested, for reasons given, that none was satisfactory for the determination of α‐linked glucose polymers in biological materials. Therefore, a method was devised in which the specific hydrolysis of starch completely to glucose is achieved by means of an amyloglucosidase. Glucose is estimated using glucose oxidase. Contents of α‐linked glucose polymers in a number of biological samples were determined, and compared with those obtained using 0.36 N‐H 2 SO 4 to effect hydrolysis.