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Site-directed mutagenesis by overlap extension using the polymerase chain reaction
Gene · 1989 · Vol. 77(1) · pp. 51–59
CRISPR and Genetic EngineeringAnimal Genetics and ReproductionMolecular Biology Techniques and ApplicationsBiologyOverlap extension polymerase chain reactionMutagenesisGeneticsSite-directed mutagenesisPrimer (cosmetics)Primer extensionDirected evolutionPolymerase chain reactionPolymerase
MeSH terms
Amino Acid SequenceAnimalsDNA-Directed DNA PolymeraseDNA, RecombinantExonsGene AmplificationGenes, MHC Class IGenes, SyntheticGenetic EngineeringMolecular Sequence DataMutationOligodeoxyribonucleotidesTaq PolymeraseMice
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