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3D pancreatic carcinoma spheroids induce a matrix-rich, chemoresistant phenotype offering a better model for drug testing

BMC Cancer · 2013 · Vol. 13(1) · pp. 95–95

Paola Longati✉(Karolinska Institutet)Xiaohui Jia(Karolinska Institutet)Johannes Eimer(Karolinska Institutet)Annika Wagman(Karolinska Institutet)Michael‐Robin Witt(Axcentua (Sweden))Stefan Rehnmark(Axcentua (Sweden))Caroline S. Verbeke(Karolinska Institutet)Rune Toftgård(Karolinska Institutet)Matthias Löhr(Karolinska Institutet)Rainer Heuchel(Karolinska Institutet)

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Abstract

We developed a high-throughput 3D cell culture drug screening system for pancreatic cancer, which displays a strongly increased chemoresistance. Features associated to the 3D cell model are increased expression of matrix proteins and miRNA as well as stromal markers such as PPP1R1B and SNED1. This is supporting the concept of cell adhesion mediated drug resistance.

3D Printing in Biomedical Research Pancreatic and Hepatic Oncology Research Cancer Cells and Metastasis3D cell cultureCancer researchSpheroidCell cultureStromal cellGemcitabinePancreatic cancerTranscriptomeMedicineCancer

MeSH terms

AnimalsAntineoplastic AgentsDisease Models, AnimalDrug Screening Assays, AntitumorEnergy MetabolismHumansPancreatic NeoplasmsPhenotypeTumor Cells, CulturedExtracellular Matrix ProteinsSpheroids, CellularDrug Resistance, NeoplasmLactic AcidCarcinoma, Pancreatic DuctalCell Line, Tumor