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A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay

Journal of Immunological Methods · 1994 · Vol. 170(2) · pp. 211–224

S. Ansar Ahmed✉(Virginia–Maryland College of Veterinary Medicine)Robert M. Gogal(Virginia Tech)Jane E. Walsh(Virginia Tech)

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Monoclonal and Polyclonal Antibodies Research Immunotherapy and Immune Responses Immune Cell Function and InteractionThymidineMolecular biologyConcanavalin ACell cultureCell growthBiologyFlow cytometryAntigenBiochemistryIn vitro

MeSH terms

AnimalsAntibody FormationCell LineCell SurvivalCells, CulturedConcanavalin AColoring AgentsFlow CytometryHybridomasKineticsLymphocyte ActivationMacrophagesMice, Inbred C57BLMonocytesMultiple Myeloma

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1,354

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References

Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens

Journal of Immunological Methods · 1984 · 655 citations

Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays

Journal of Immunological Methods · 1983 · 55,054 citations

An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT

Journal of Immunological Methods · 1991 · 1,010 citations

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