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Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry

Journal of Proteome Research · 2003 · Vol. 2(5) · pp. 488–494

Tomoyoshi Soga✉(Kyoto University)Yoshiaki Ohashi(Keio University)Yuki Ueno(Kyoto University)Hisako Naraoka(Keio University)Masaru Tomita(Kyoto University)Takaaki Nishioka(Keio University)

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Abstract

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.

Metabolomics and Mass Spectrometry Studies Microbial Metabolic Engineering and Bioproduction Advanced Proteomics Techniques and ApplicationsMetabolomeCapillary electrophoresisMass spectrometryChromatographyCapillary electrophoresis–mass spectrometryBacillus subtilisMetabolomicsChemistryQuantitative analysis (chemistry)Biology

MeSH terms

AnionsBacillus subtilisBacterial ProteinsCationsCoenzyme AGlycolysisModels, ChemicalNucleotidesMass SpectrometrySpores, BacterialElectrophoresis, Capillary

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972

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