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DNA sequencing with chain-terminating inhibitors
Proceedings of the National Academy of Sciences · 1977 · Vol. 74(12) · pp. 5463–5467
Frederick Sanger✉(MRC Laboratory of Molecular Biology)S. Nicklen(Medical Research Council)Alan Coulson(Medical Research Council)
Abstract
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.
Bacteriophages and microbial interactionsRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyDNASanger sequencingNucleotideDNA polymerasePolymerase chain reactionChemistryDNA sequencingBacteriophagePolymeraseMolecular biology
MeSH terms
Base SequenceColiphagesDeoxyribonucleotidesDNA Polymerase IDNA Restriction EnzymesDNA, ViralMethods
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References
A new method for sequencing DNA.
Proceedings of the National Academy of Sciences · 1977 · 7,962 citations
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
Journal of Molecular Biology · 1975 · 2,682 citations
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