Extraction, separation and identification of active compounds in Rosemarinus officinalis by using thin layer chromatographic (TLC) and gas chromatography and mass spectrometry studies

Abstract

Sohxlet apparatus is used to prepare the plant extract from the leaves of Rosemarinus officinalis by using the solvent of hexane. TLC with hexane solvent of R. officinalis gave 5 spots, and the active compounds were isolated. R. officinalis spots was eluted through TLC employed in GCMS found the presence of 22 active compounds like Eucalyptol, Borneol, Camphor, trans-á-Terpinyl pentanoate, D-Verbenone, Bornyl acetate, Caryophyllene, à-Caryophyllene, Naphthalene, Cedrene, Copaene, trans-Z-à-Bisabolene epoxide, 1-Heptatriacotanol, n-Hexadecanoic acid, Heptamethyl-3-phenyl-1,4-cyclohexadiene, Trenbolone Acetate, 9,12-Octadecadienoyl chloride, (Z,Z)-, and other few unidentified compounds were identified.