In vitro anti-helminthic, antimicrobial, antioxidant, activity and GC-MS of fractions of the stem bark of Memecylon accedens

Abstract

The three fractions, F1, F2, and F3 obtained from TLC-guided bulking were tested for antimicrobial activity by broth microdilution method using the following microbes: Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Salmonella typhi, and Candida albicans. F1 demonstrated the highest activity in the antimicrobial analysis, followed by F3, and F2 had the least activity. Anthelmintic analysis of extract using Pheretima posthuman showed promising activity. The fractions also exhibited activity with F1 having the highest activity. DPPH radical scavenging was performed for the fractions to test for antioxidant activities. The IC50 values recorded for extract, F1, F2, and F3 DPPH assay were 54.46±0.104 µg/ml, 42.53±0.238 µg/ml, 115.44±0.496 µg/ml, and 45.23±0.058 µg/ml respectively. F1 showed the greatest antioxidant activity. The fractions were also subjected to FT-IR and GC-MS analysis. This was done to obtain information to confirm the phytochemicals that were present in the extract and each of the fractions. The findings of this study provide insights into the possible use of Memecylon accedens as a potential agent for the discovery and development of drugs for the treatment of Helminth-related diseases, reactive oxidants, and microbial infections.