Antioxidant potential and assessment of subacute toxicity of an ivorian medicinal recipe

Abstract

Recipes based on medicinal plants are used to treat a variety of ailments. Indeed, these plants seem to be the reservoir of new active ingredients. However, although they have proven pharmacological properties, many side effects are attributed to herbal remedies. The aim of this study was to evaluate the In vitro antioxidant potential and acute and subacute toxicity of a medicinal recipe used in the treatment of malaria. To this end, the In vitro antioxidant activity of the extract was assessed by DPPH and ABTS tests. The acute toxicity test of Ahoutou and the optimized extract were evaluated on groups of mice. For sub-acute toxicity, four groups of 4 rats were orally administered the different extracts at doses of 60mg/Kg-bw, 120 mg/Kg-bw and 180 mg/Kg-bw per day for 28 days. The results show that the phytochemical studies revealed the presence of bioactive chemical families. We also noted the presence of certain important polyphenolic compounds such as catechin, gallic acid and quercetin, which are powerful antioxidants. Evaluation of antioxidant activity In vitro showed that the Ahoutou recipe and its optimized extracts were capable of reducing DPPH and ABTS free radicals. Acute and sub-acute toxicity tests showed that Ahoutou and its optimized extracts are non-toxic by the oral route, and have no deleterious effects on kidney, liver or heart tissue. Ahoutou and its optimized extracts could be a scientific basis for the development of natural antioxidant agents, and therefore the establishment of new therapeutic molecules.