Antimitotic and free radical scavenging properties of a herbal preparation used in the treatment of fibroid

Abstract

The research aimed at evaluating the antimitotic and antioxidant property of a herbal preparation and its component plant used in the treatment of fibroid. Phytochemical and physicochemical parameters were assessed using standard methods. Allium cepa anti-mitotic assay was used for anti-proliferative investigation of the aqueous methanol extract of the herbal powder and Rauwolfia vomitoria fruits at a concentration range of 1.25-20 mg/mL. Vincristine (0.01 mg/mL) was used as positive control. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to assess free radical scavenging activity. Flavonoids, Tannins, carbohydrates, phenols and terpenoids were present in the products. The product showed 3.1, 2.4, 0.45, 5.5 and 1.4% value in total ash, acid insoluble ash, water soluble ash, sulphated ash, and moisture content respectively. The aqueous extract of the herbal powder and R. vomitoria demonstrated a dose and time-dependent anti-mitotic activity with complete inhibition of mitosis. The IC50 of 17.0 µg and 62.2 µg for ascorbic and the herbal preparation respectively on DPPH was obtained. The study suggests that the anti-fibriod herbal drug have antioxidant and Antimitotic potentials.