Bacteriological assessment of lactic acid bacteria in the last stage effluents from cassava and Ogi production

Abstract

The objective of this work was to isolate and characterize Lactic acid bacteria from Cassava (Fufu) and Ogi Effluents. A total of two hundred effluents (Consisting of 100 each from Ogi and cassava during processing), were randomly collected in a 10 ml wide-mouthed sterile sample container from processing homes in Ezza-North L.G.A for 20 days. The collected samples were aseptically analyzed using standard microbiological methods. The results showed that the lactic acid bacterial counts of the effluents generated from ogi and cassava fufu production range from 7.0×104 to 8.8×105. Ogi effluents accounted for 113 LAB comprising Lactiplantibacillus argentoratensis (n=20), Lactococcuslactis (n=24), Lactiplantibacillus plantarum (n=24), Leuconostoc pseudomesenteroides (n=14), Leuconostoc mesenteroides (n=16) Pediococcus pentosaceus (n= 15). Fufu effluents accounted for 122 LAB comprising Lactiplantibacillus argentoratensis (n=25), Lactococcus lactis (n=26), Lactiplantibacillus plantarum (n=19), Leuconostoc pseudomesenteroides (n=20), Leuconostoc mesenteroides (n=15) Pediococcus pentosaceus (n= 17). It also revealed Ogi and fufu processing effluents as suitable sources of new LAB such as Leuconostoc pseudomesenteroides and Pediococcus pentosaceus which have not been identified in related studies from Nigeria. Therefore an in vivo and in vitro probiotic characteristic of these potential Lactobacillus strains is encouraged.