Estimation of the minimum inhibitory concentration (MIC) of the ethanolic extract of S. monoica as an antifungal agent for Candida albicans

Abstract

Medicinal plants have shown an important role in treating many different diseases, and the world still depends on many of these plants in primary and advanced health care. These plants have been used as important sources for treating many types of pathogenic fungi. In the current study, the leaves of the S. monoica plant were extracted using absolute ethanol (99%). The components of the ethanolic extract of the leaves of the S. monoica plant were analyzed using the GC-Mass technique. The effectiveness of this extract was evaluated and the value of the minimum inhibitory concentration (MIC) was estimated as an antifungal agent. Candida albicans species. The results showed that the ethanolic content of S. monoica leaves contains many active compounds, including: Stigmasterol, 9,12,15-Octadecatrienoic acid, 2,3-dihydroxypropyl ester, (Z, Z, Z), Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl, 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one, Pterin -6-carboxylic acid, Glycine, N,N-dimethyl-methyl ester, Acetyl turicine, 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran- 2(4H)-one, Phenol, 2,2'- methylenebis[6-(1,1- dimethylethyl)-4- methyl. The results also showed that the ethanolic extract has the ability to inhibit the growth of Candida albicans fungi. It was observed that the diameters of inhibition of the fungal colonies decreased with the increase in the concentration of the ethanolic extract used, as the value of the minimum inhibitory concentration of the ethanolic extract reached 0.8 mg/ml towards these fungi. Therefore, it is possible to use the S. monoica plant as a natural source of treatment for many disease-causing fungi.