Oocyte isolation techniques from the ovary samples of slaughtered animals: A review

Abstract

The isolation of oocytes from the ovaries is the basic and essential step for In vitro Embryo Production. The oocyte recovery techniques differ between the live and slaughtered source of animals and the present review focuses on the recovery methods of abattoir ovary samples. The oocyte retrieval techniques include slicing, aspiration, puncture, dissection, and other techniques that are individually efficient in this process. The oocyte isolation techniques are associated only with the characteristics of quantity, and quality of oocytes and the characteristics directly influence the oocyte/ embryo developmental competence and gross IVEP. Based on the choice of laboratory and availability of Sample size the oocyte recovery methods are used and every oocyte isolation technique has attained its own pros and cons still there is no perfect technique has been invented in the reproductive field.