Steap-3 gene expression profiling in ischemic and dilated cardiomyopathy rat models

Abstract

Background: Despite the implementation of contemporary therapeutic approaches, individuals diagnosed with heart failure have a mere 50% survival rate over a five year period. In order to enhance the advancement of novel treatment approaches, it is imperative to utilize preclinical disease models that accurately replicate the complexities of the human situation. The relationship between aberrant iron regulation, myocardial function, and prognosis in heart failure is established in the scientific literature. However, there is a lack of comprehensive understanding about the expression patterns of genes associated with iron regulation, both at the tissue level of the myocardium and at the level of individual cells. Objectives: Measuring differential gene expression of STEAP3 gene in animal models of heart failure VS. Control. Methodology: Quantitative polymerase chain reaction (qPCR) was used to measure gene expression profiling of STEAP3 gene in blood and left ventricular tissue samples of circumflex artery ligation ischemic injury rat models and doxorubicin induced dilated cardiomyopathy rat models VS. control rats. Results: STEAP3 gene was significantly downregulated in both rat heart failure models compared to control rats. Conclusion: STEAP3 gene is significantly downregulated in heart failure animal models.