Evaluation of minimum inhibitory concentration of Glycyrrhiza glabra and Zingiber officinale against different bacteria

Abstract

The present study was conducted to study the antibacterial activity of fresh rhizome aqueous extract of Glycyrrhiza glabra and Zingiber officinale against Escherichia coli, Klebsiella, and Staphylococcus spp. In vitro minimum inhibitory concentration of extracts against test organisms were determined. The research was conducted at Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur Madhya Pradesh (M.P.). The aqueous extract of Glycyrrhiza glabra and Zingiber officinale was prepared and were tested on isolates of Escherichia coli, Klebsiella, and Staphylococcus spp. with the help of standard tube dilution method. Antibacterial activity of the aqueous extract of Zingiber officinale rhizomes showed minimum inhibitory concentration of 12.5 mg/ml, 12.5 mg/ml and 50 mg/ml against bacteria E. coli, Klebsiella spp. and Staphylococcus spp. respectively. The aqueous extract of combination of Glycyrrhiza glabra and Zingiber officinale rhizomes showed minimum inhibitory concentration of 25 mg/ml, 25 mg/ml and no inhibition against bacteria E. coli, Klebsiella spp. and Staphylococcus spp. respectively. The study indicated that Zingiber officinale plant can act as a potential source of antibacterial agent.