In-depth analysis of deleterious non-synonymous SNPs in the bovine ANPEP gene and their structural consequences

Abstract

This study delves into the genetic variations within the Aminopeptidase N (ANPEP) gene in cattle, focusing on missense variants and their potential impact on protein structure, stability, and function. A dataset containing SNPs was retrieved from Ensembl-Biomart and Uniprot databases. Deleterious non-synonymous SNPs were identified using SIFT, PANTHER, POLYPHEN-2, PhDSNP, PredictSNP, and SNAP2. The study also predicts the structural and functional consequences of these SNPs using I-Mutant 2.0, MUPRO, mCSM, and HOPE. Furthermore, protein-protein interaction analysis was conducted using STRING. Results reveal a set of common deleterious variants with potential implications for protein stability. The study enhances our understanding of genetic variations in the ANPEP gene in cattle, offering insights into potential functional consequences.