Molecular detection of Salmonella in broiler chicken

Abstract

Salmonella is one of the infectious diseases that can found in broiler chicken. Salmonella is also a potential pathogen that can cause disease in both animal and human. This study was designed as reference for detection and identification of Salmonella bacteria from broiler chicken feces using culture methods and combined with PCR analysis. 10 fecal samples were isolated from close house type farm at Tabanan district, Bali, Indonesia. The samples are transported using Stuart media, enriched using Selenite broth and cultured in Xylose-lysine-deoxycholate (XLD) agar. After the Salmonella colonies grow, the DNA are extracted, proceed to PCR analysis and sent for sequencing. The result showed that 60% of the samples are Salmonella enterica bacteria that confirmed by culture media and molecular method.