Determination of cyromazine and Melamine residues in poultry eggs

Abstract

Cyromazine (CYR) and Melamine residues (MEL) in 40 samples of eggs were detected from layer farms located in selected districts of Haryana (Ambala, Jind and Panipat) using. High Pressure Liquid Chromatography (HPLC-UV). The separation was achieved using C18 column using acetonitrile and acidified TFA as a mobile phase (0.05%) with a flow rate of 1 ml/minute with UV detection at wavelength 214 and 230 nm at column temperature of 28 °C. Out of these, CYR was detected in 03 samples above the LOD (5.3 μg/kg). MEL was not detected in any of the sample analyzed. Cyromazine in the eggs can appear if the layer is subjected to fed cyromazine contaminated feed deliberately.