Quantitative determination of phytochemical constituents in seeds of common methi and Kasuri methi

Abstract

Phytochemicals are chemical compounds produced by plants and play role in resistance against bacteria, fungi, and virus infections. Medicinal and spice crops are known to be rich in phytochemicals. Fenugreek is a multipurpose crop and is also considered Generally Recognised as Safe (GRAS) by the FDA. In order to estimate the phytochemicals abundance in different genotypes of fenugreek using seeds samples of methi (Trigonella foenum-graecum L.) (RMT-305, AFG-1, Pusa Early bunching) and Kasuri methi (Trigonella corniculata L.) were investigated for their potential phytochemicals abundance such as phenols, flavonoids, tannins, alkaloids and proteins by spectrophotometer method. Among genotypes, kasuri methi as found highest phytochemical contents such as phenol (71.80 mg/100 gm), flavonoids (90.28 mg/gm), tannins (244.53/gm), alkaloids (42.64/gm) and protein (55.10/gm) followed by RMT-305, AFG-1, and Pusa Early bunching. These findings showed that Kasuri methi had a significant quantity of phytochemicals. Further, such research outcomes useful for confirmation of effectiveness of phytochemicals on plant pathogens by using bioassay.