Evaluation of anti-oxidant, anti-nociceptive, CNS depressant and antidiarrheal activities of Rorippa palustris leaf extract

Abstract

This study aimed to examine certain in vitro and in vivo characteristics of plant extracts obtained from Rorippa palustris through the use of methanol extraction. The principal mode of evaluation for the methanolic extract of Rorippa palustris (MERP) was established to investigate the plant's in vitro potential for activities such as antioxidant, antinociceptive, neuropharmacological, and antidiarrheal activity. This is due to the pharmaceutical interest in the constituent parts of the plant. The DPPH Free Radical Scavenging Assay was utilized to measure antioxidant activity across multiple doses. The hot plate method, acetic-acid-induced writhing test, and glutamate-induced mechanism test were employed to conduct in vivo antinociceptive testing. The study conducted a neuropharmacological experiment utilizing the hole cross and open field methods, as well as an anti-diarrheal test employing the magnesium sulfate-induced diarrheal test. The IC50 value of 160.04 µg/mL has demonstrated the antioxidant activity of MERP, as per the results of the conducted research. The results of the hot plate test and the acetic acid-induced writhing test indicated a noteworthy antinociceptive effect, with a percentage of inhibition of 46.2% and 66.61% observed at doses of 200 and 400 mg/kg, respectively. The assessment of the antinociceptive activity mechanism(s) of the glutamate-induced writhing test has yielded noteworthy outcomes, with a substantial percentage of inhibition recorded at 52.35% and 75.93%. The neuropharmacological examination has exhibited potential efficacy, while the antidiarrheal evaluation has demonstrated 80.15% and 85.00% inhibition rates. In conclusion, it is evident that the phytochemical present in this plant exhibits potent pharmacological properties, making it a promising candidate for drug discovery in various fields.