Comparative antibacterial activity of orange seed oil and antibiotics of choice against some food borne pathogens

Abstract

In this present study, crude antibacterial potential of citrus seed oil was investigated against some selected food borne pathogens (Bacillus cereus, Klebsiella sp., E.coli, Staphylococcus aureus and Pseudomonas sp.) using standard procedures. The antibacterial activity (zones of inhibition (mm) ranged from 6.3±0.03-11.00±0.0 with E.coli and Pseudomonas sp. been the most resistant and Staphylococcus sp. been the most susceptible. The MIC assay showed that the oil at 6.25 and 3.12mg/ml only had effect on Bacillus sp. Staphylococcus sp. and Klebsiella sp. At 12.5mg/ml, all the test organisms were susceptible. The orange seed oil compared slightly with the antibacterial activity of antibiotic of choice. Antibacterial efficacy shown by the seed oil provides a scientific basis and thus validates their use as medicinal remedies. Isolation and purification of different phytochemicals may further yield significant antibacterial agents.