Standardization of callus induction in danthapala (Wrightia tinctoria R.Br.)

Abstract

Wrightia tinctoria R.Br. (Family: Apocynaceae) commonly called “Indrajau” is distributed throughout the world and occurs abundantly in India. It is a deciduous tree with white fragrant flowers. The seeds and bark of this plant are used in Indian traditional medicine as anti-diarrheal, anti-dysenteric and also for the treatment of psoriasis. Callus induction is an important stage in micropropagation and was largely influenced by the medium, plant growth regulator, and explant.The present study was conducted at Onattukara Regional Agricultural Research Station, KAU, Kayamkulam to standardise the media for callus induction. The results revealed that callus induction from nodal segments started 20 days after inoculation and was high in MS medium containing BA 1 mg l-1 and 2,4-D 1.5 mg l-1. The callusing from the leaf started within 25 days after inoculation. It shows best in MS medium containing 1 mg l-1 NAA and 2 mg l-1 2,4-D.