Assessment an antibacterial activity of crud bodies, Ailolopus thaiossinus (Orthoptera) and Polistes watti larvae (Hymenoptera) by extracted cold and boiled solvents

Abstract

Todays, insects as adults and immaturs are in focusing of researching new pharmaceutical compounds. Especially, those under habit and habitat stressors. However, insect body extracts have antibacterial activity to current antibiotic resistant bacteria. After the extraction by sequential solvent method, bacterial growth inhibition of dry bodies the insects: grass hopper, Ailolopus thaiossinus, larvae of paper wasp, Polistes watti and standard antibiotic drug Ceftriaxone (CRO) as positive control against the following bacteria species: Gram - positive Staphylococcus aureus and Gram - negatives; Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae have been tested. Antibacterial parameter was tested by disc diffusion test. Only E. coli and K. pneumoniae were sensitive to hexane extract with A. thaiossinus and P. watti larvae respectively. The two extracts with diethyl ether were exhibited moderate growth inhibition of the tested bacteria except for P. aeruginosa resistant to larvae P. watti extract. Extracts with ethyl acetate were varied between moderate to resistant activity, Later, methanol extract of the tested insect materals were more effective than the standard drug (CRO) for S. aureus, P. aeruginosa and K. pneumoniae. The boiled methanol extract of A. thaiossinus more effective than ones of larvae P. watti and cold extract of A. thaiossinus. In alternative antibiotics, this study as bricks in this field, through practical application the antibacterial compounds as templates for future antibiotic generation.