Standardisation of anthocyanin extraction techniques from hibiscus (Hibiscus rosa-sinensis) petals for biocolour utilisation

Abstract

In recent years, the use of natural anthocyanin as a biocolour in business has grown to peak and there is an increasing desire to enhance the extraction procedures. In this study, various extraction techniques (Conventional extraction, Microwave assisted extraction and Ultrasound assisted extraction) for extracting anthocyanin from Hibiscus rosa-sinensis petals using water, ethanol, and citric acid as solvents have been investigated. Total Anthocyanin, Total Antioxidant, Total Phenolic Content, Total Flavonoid, Total Soluble Solids, Titrable Acidity and pH are estimated in this study. The results showed that Ultrasound assisted extraction recorded higher anthocyanin rate (179.32mg/l) when compared to Microwave assisted extraction (155.45mg/l) and Conventional extraction (100.88mg/l).This study also showed that extraction efficiency is more significant in aqueous solvent in Ultrasound assisted extraction rather than other solvents.