Development, characterization and in vitro antifungal evaluation of topical formulation of Tridax procumbens L. Leaf extract

Abstract

Background: Fungal infections have become major concern and are caused by various fungi. The primary target of these fungi is skin which leads to numerous skin disorders like eczema, dermatitis, candidiasis, athlete’s foot, ringworm etc. Objective: To develop a safe and effective antifungal topical cream formulation of Tridax procumbens L. leaf extract. Method: The ethanolic extract was prepared using soxhlet apparatus. The phytochemical screening and molecular docking studies of extract was performed. The cream was prepared by oil in water emulsion method. MIC studies was carried out by Agar dilution method and antifungal activity of cream was carried out by Agar diffusion method against Candida albicans. Results: Phytochemical screening of extract revealed the presence of various phytoconstituents. Docking results indicated benzamide derivative showing best protein-ligand interactions. The extract of Tridax procumbens L. showed potent antifungal activity with MIC value 0.88 mg/ml. The cream also exhibited significant antifungal activity.