Development of diagnostic method for marine fish pathogens

Abstract

Rapid detection of bacterial fish pathogens in aquacultureis very important foreffective disease controlin Turkey which has total productioncapacity of 240 thousand tons of culturedfish. Conventional microbiological methods are time consuming and lack sensitivities to detect latent marine pathogens. The application of new biotechnologic methods for detection will make a significant impact on the development of such rapid diagnostic approach to aquaculture. In this study, we have used gene based PCR methods to developed rapid diagnostic kit for fast detection of bacterial infection in marine fish. Four different modified oligonucleotid primers (vvhA, aero, gyrB and hsp70) have designed to detect of Vibrio spp., Aeromonas spp., Pseudomonas spp., and Mycobacteria spp. from marine fish. As a result of this study, four different bacterial agents Aeromonas salmonicida, Vibrio anguillarum, Pseudomonas anguilliseptica and Mycobacterium marinum simultaneously has been identified and detected with using 8 primers in same-single tube in very short time.