Effect of methanol extracts from Salvia purpurea CAV. on the proliferation of bacteria and hematopoietic cells

Abstract

The effect of the methanolic extract of Salvia purpurea Cav. (Labiatae) was evaluated. Consecutive macerations were prepared with the hexane, dichloromethane, methanol, and water of three mixtures of the aerial structures: flower, flower–leaf, and flower–leaf–stem. The antibacterial activity was assessed by the method of resazurin. The bacteria employed were: Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Shigella flexneri, Salmonella typhi, and Proteus mirabilis. Hematopoietic activity was evaluated in bone-marrow and spleen mouse cell cultures utilizing 1, 10, and 100 µg/mL. The extracts of flower and flower–leaf presented a minimal inhibitory concentration (MIC) of 0.33 µg/mL vs. S. typhi and B. subtilis, respectively. The flower–leaf extract stimulated better cell proliferation, increasing by 166, 178, and 171% in the cellularity with 1, 10, and 100 μg/mL, respectively, vs. the control (p