Molecular characterization of chickpea (Cicer arietinum L.) Genotypes using ISSR markers

Abstract

Chickpea (Cicer arietinum L., 2n=16) is a legume of family Fabaceae, subfamily Faboideae. Chickpea is the second largest grown food legume of the world (Gaur et al., 2008). Genetic divergence among 28 genotypes of Cicer arietinum L. was assessed through ISSR molecular markers. ISSR analysis showed 85.09 per cent of polymorphism. A total of 109 amplified bands were obtained that ranged between 150 bp to 2100 bp using 20 ISSR primers. The 92 out of 109 bands were polymorphic. The average PIC (polymorphic information content) of ISSR was found 0.257. Jaccard’s similarity coefficient for ISSR lay between 0.54 to 0.94 (54% to 94%). The similarity coefficients values were significant for this marker. Dendogram based on genetic distance calculated by UPGMA method segregated the 28 chickpea genotypes into three ISSR major clusters, respectively. Two and three dimension principal component analysis (PCA) showed similar clustering of 28 genotypes as evident from cluster tree analysis with dice similarity coefficients ranged from 0.77 to 0.91 (77% to 91%)for ISSR analysis. Based upon ISSR analysis of 28 genotypes with 20 primers genotype PBC-509, PBC-511, Pratap channa-1, Dohad yellow and Pratap Rajchana were identified as genetically diverse. Therefore, the results of the present study can prove helpful for implementation of chickpea improvement programmes.