Survey and isolation of Trichoderma spp. from rhizosphere soils of Western Maharashtra

Abstract

In this study, the total 45 samples were collected from areas of Western Maharashtra. Out of these only 20 rhizospheric soil samples had the population of Trichoderma spp. The Trichoderma was isolated from the soil samples collected from the different locations of western Maharashtra by serial dilution technique and plating method. By visual observations 6, T. hamatum, 7, T harzianum (Rifai), 3, T. koningii (Oudem) and 4, T. asperellum (Samuels) were identified. T. harzianum and T .hamatum were present in most of the soil samples collected from Western Maharashtra region. The T. asperellum species was obtained from soil samples collected from Dhabewadi, sakri, Pachora and Tasgaon region of western Maharashtra. These isolates of Trichoderma spp. were studied and identified on basis of macroscopic i.e. colony characters like growth rates, pigmentation, colony edge, pustule formation showing defined characteristics for that respective species.